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. 1974 Sep;71(9):3522–3526. doi: 10.1073/pnas.71.9.3522

A Ca++-Dependent and -Selective Ionophore as Part of the Ca++ + Mg++-Dependent Adenosinetriphosphatase of Sarcoplasmic Reticulum

Adil E Shamoo *, David H MacLennan †
PMCID: PMC433806  PMID: 4279408

Abstract

Solubilized Ca++ + Mg++-dependent adenosinetriphosphatase (EC 3.6.1.3; ATP diphosphohydrolase) from sarcoplasmic reticulum increased bimolecular lipid membrane (oxidized cholesterol) conductance several hundred-fold. The relative conductance change and the relative permeability elicited by this material has the following sequence: Ba++ > Ca++ > Sr++ > Mg++ > Mn++ > Zn++, Na+, K+, Cs+, Li+, and Rb+. Zn++ and Na+ strongly inhibit the increase in Ca++ conductance obtained with solubilized Ca++ + Mg++-dependent adenosinetriphosphatase. The Ca++-ionophore is an integral part of the Ca++ + Mg++-dependent adenosinetriphosphatase enzyme and may function as a Ca++-carrier in the overall Ca++-pump of sarcoplasmic reticulum.

Keywords: bimolecular lipid membrane, permeability, conductance

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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