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. 1974 Sep;71(9):3527–3531. doi: 10.1073/pnas.71.9.3527

The Localization of Transfer RNA5Lys Genes in Drosophila melanogaster

T A Grigliatti *, B N White *, G M Tener *, T C Kaufman , D T Suzuki
PMCID: PMC433807  PMID: 4215081

Abstract

Transfer RNA5Lys was isolated and purified from Drosophila melanogaster. It was iodinated in vitro with sodium [125I]iodide and hybridized in situ to the polytene salivary gland chromosomes from Drosophila. Autoradiographs exposed three to four weeks allowed the localization of this tRNA to the right arm of chromosome 2 between bands 48F and 49A.

Keywords: 5S RNA, 125I-iodination, in situ hybridization

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Selected References

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