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. 1974 Sep;71(9):3550–3554. doi: 10.1073/pnas.71.9.3550

The Role of the Skin in Active Specific Immunization Against Leukemia in Guinea Pigs

Ludwik Gross 1, Yolande Dreyfuss 1
PMCID: PMC433812  PMID: 4139717

Abstract

The L2C leukemia strain, which originated as spontaneous leukemia in “strain 2” guinea pigs, is transmissible by cell-graft in animals of this line; on subcutaneous inoculation it induces consistently generalized and progressive stem-cell leukemia in 99% of the inoculated animals. The leukemia thus induced never regresses. However, when very small doses of leukemic cell suspensions (0.05 ml of a 10-6 or 10-7 dilution) were inoculated intradermally, 86 out of 180 intradermal tumors (48%) regressed spontaneously. Most of the animals that recovered from the intradermal tumors were resistant to a challenging reinoculation of leukemic cells. This resistance could be substantially increased by a second intradermal inoculation of leukemic cells. Females were more resistant than males. When 55 immunized females and 36 males received a challenging subcutaneous reinoculation (0.5 ml each) of a leukemic cell suspension of 10-2 dilution, only two females and six males developed leukemia; the remaining 83 animals (91%) remained in good health. In a control experiment, 126 untreated “strain 2” guinea pigs were inoculated subcutaneously with the same dose, and all but one (99%) developed leukemia. The immunity thus induced could not be transferred to other animals by a serum collected from immunized guinea pigs.

Keywords: intradermal immunization, skin and tumor immunity, virus in guinea pig leukemia

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CONGDON C. C., LORENZ E. Leukemia in guinea-pigs. Am J Pathol. 1954 Mar-Apr;30(2):337–359. [PMC free article] [PubMed] [Google Scholar]
  2. Feldman D. G., Gross L. Electron microscopic study of the guinea pig leukemia virus. Cancer Res. 1970 Nov;30(11):2702–2711. [PubMed] [Google Scholar]
  3. Gross L. Attempt to immunize guinea pigs against leukemia by skin scarification with leukemic cell suspensions. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3432–3436. doi: 10.1073/pnas.70.12.3432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gross L., Dreyfuss Y., Ehrenreich T., Moore L. A. Experimental studies on leukemia in guinea pig. Acta Haematol. 1970;43(4):193–209. doi: 10.1159/000208730. [DOI] [PubMed] [Google Scholar]
  5. Gross L., Feldman D. G., Ehrenreich T., Dreyfuss Y., Moore L. A. Comparative studies of subcutaneous and intradermal leukemic tumors in guinea pigs. Cancer Res. 1973 Feb;33(2):293–301. [PubMed] [Google Scholar]
  6. Gross L. Specific, active, intradermal immunization against leukemia in guinea pigs. Acta Haematol. 1970;44(1):1–10. doi: 10.1159/000208656. [DOI] [PubMed] [Google Scholar]
  7. Gross L. Studies on the nature of acquired immunity against leukemia in guinea pigs. Acta Haematol. 1971;45(4):218–231. doi: 10.1159/000208628. [DOI] [PubMed] [Google Scholar]
  8. JUNGEBLUT C. W., KODZA H. Studies of leukemia L2C in guinea pigs. Arch Gesamte Virusforsch. 1963;12:537–551. doi: 10.1007/BF01242160. [DOI] [PubMed] [Google Scholar]

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