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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Sep;71(9):3560–3564. doi: 10.1073/pnas.71.9.3560

The Nucleotide Sequence Complexity of Avian Tumor Virus RNA

M A Billeter 1, J T Parsons 1,*, J M Coffin 1
PMCID: PMC433814  PMID: 4372625

Abstract

The nucleotide sequence complexity of 60-70S RNA of Rous sarcoma virus was determined from the molar yields of 11 pure oligonucleotides of known chain length, obtained from [32P]RNA of Rous sarcoma virus by T1 RNase digestion and two-dimensional polyacrylamide gel electrophoresis. We calculate an apparent chain length of about 9800 nucleotides, corresponding to a molecular weight of 3.4 × 106. Assuming the molecular weight of 60-70S RNA of Rous sarcoma virus to be about 107, our data suggest that the virus genome consists of identical, or very similar, nucleotide sequences repeated three to four times.

Keywords: Rous sarcoma virus, T1-oligonucleotides, two-dimensional gel electrophoresis

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Selected References

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