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. 2015 Feb;21(2):262–278. doi: 10.1261/rna.048090.114

FIGURE 4.

FIGURE 4.

PROMPTs are accumulating by anisomycin and partially by LPS stimulations in monocytic THP-1 cells. (A) THP-1 cells were stimulated for 1 h with 10 µg/mL anisomycin in the presence or absence of the p38-inhibitor BIRB796 and the indicated proteins were assayed by Western blot. (B) THP-1 cells were stimulated for 2 h with 10 µg/mL anisomycin in the presence or absence of the p38-inhibitor BIRB796. Consequently, PROMPTs RNA levels were determined by qRT-PCR and shown as x-fold induction relative to the nonstimulated expression levels. (C) LPS stimulation (1 µg/mL) for 1 h of THP-1 cells resulted in a strong induction of TNFα mRNA in THP-1 cells (right panel). Induction of TNFα was sensitive to inhibition of p38MAPK activity. Blotting for endogenous proteins showed similar loss of RBM7 band intensity as in Figure 1E–G or A. (D) The same cDNAs as in C were analyzed for PROMPTs RNA levels. While proRBM39, proEXT1, and proDNJAB4 levels were not changed, proIFNAR1 levels were twofold up-regulated by LPS stimulation in a p38-dependent manner. All graphs are displayed with standard deviations. The star indicates P < 0.04 (as determined by two-sided Student's t-test) for the LPS-induced up-regulation of proIFNAR1.