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. 1974 Sep;71(9):3672–3676. doi: 10.1073/pnas.71.9.3672

Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors

Mary-Dell Chilton *, Thomas C Currier *, Stephen K Farrand *, Arnold J Bendich †, Milton P Gordon ‡, Eugene W Nester *
PMCID: PMC433838  PMID: 4530328

Abstract

Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 104-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per three diploid tumor cells).

By the same technique, PS8 bacteriophage DNA is not detected in crown gall tumor DNA under conditions that allow detection of 0.0007-0.001% added phage DNA. Crown gall tumor DNA cannot contain as much as one entire phage genome per diploid tumor cell. The presence of a small fraction of either genome (less than 5%), even if present as multiple copies, would escape detection by this method.

Keywords: nucleic acid hybridization, DNA reassociation kinetics, transformation of plant cells

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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