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. 1974 Sep;71(9):3754–3757. doi: 10.1073/pnas.71.9.3754

Simian Virus 40 T Antigen Binds to DNA

R B Carroll 1, L Hager 1,*, R Dulbecco 1
PMCID: PMC433855  PMID: 4372636

Abstract

The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.

Keywords: sedimentation, multiple species, dissociation, denatured DNA, native DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Amodio F. J., Jenkins M., Gutmann E. D., Ferris F. L. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:289–305. doi: 10.1101/sqb.1968.033.01.033. [DOI] [PubMed] [Google Scholar]
  2. Alberts B. M. Function of gene 32-protein, a new protein essential for the genetic recombination and replication of T4 bacteriophage DNA. Fed Proc. 1970 May-Jun;29(3):1154–1163. [PubMed] [Google Scholar]
  3. BLACK P. H., ROWE W. P., TURNER H. C., HUEBNER R. J. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Dec;50:1148–1156. doi: 10.1073/pnas.50.6.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Del Villano B. C., Defendi V. Characterization of the SV40 T antigen. Virology. 1973 Jan;51(1):34–46. doi: 10.1016/0042-6822(73)90363-2. [DOI] [PubMed] [Google Scholar]
  5. FRIED M. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Mar;53:486–491. doi: 10.1073/pnas.53.3.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Francke B., Eckhart W. Polyoma gene function required for viral DNA synthesis. Virology. 1973 Sep;55(1):127–135. doi: 10.1016/s0042-6822(73)81014-1. [DOI] [PubMed] [Google Scholar]
  7. GILDEN R. V., CARP R. I., TAGUCHI F., DEFEND V. THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN. Proc Natl Acad Sci U S A. 1965 Mar;53:684–692. doi: 10.1073/pnas.53.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hinkle D. C., Chamberlin M. J. Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection. J Mol Biol. 1972 Sep 28;70(2):157–185. doi: 10.1016/0022-2836(72)90531-1. [DOI] [PubMed] [Google Scholar]
  9. Lazarus H. M., Sporn M. B., Smith J. M., Henderson W. R. Purification of T antigen from nuclei of simian virus 40-induced hamster tumors. J Virol. 1967 Oct;1(5):1093–1095. doi: 10.1128/jvi.1.5.1093-1095.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lewis A. M., Jr, Rowe W. P. Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. J Virol. 1971 Feb;7(2):189–197. doi: 10.1128/jvi.7.2.189-197.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oxman M. N., Takemoto K. K., Eckhart W. Polyoma T antigen synthesis by temperature-sensitive mutants of polyoma virus. Virology. 1972 Sep;49(3):675–682. doi: 10.1016/0042-6822(72)90524-7. [DOI] [PubMed] [Google Scholar]
  12. Potter C. W., McLaughlin B. C., Oxford J. S. Simian virus 40-induced T and tumor antigens. J Virol. 1969 Nov;4(5):574–579. doi: 10.1128/jvi.4.5.574-579.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAPP F., BUTEL J. S., FELDMAN L. A., KITAHARA T., MELNICK J. L. DIFFERENTIAL EFFECTS OF INHIBITORS ON THE STEPS LEADING TO THE FORMATION OF SV40 TUMOR AND VIRUS ANTIGENS. J Exp Med. 1965 Jun 1;121:935–944. doi: 10.1084/jem.121.6.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RAPP F., KITAHARA T., BUTEL J. S., MELNICK J. L. SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40). Proc Natl Acad Sci U S A. 1964 Nov;52:1138–1142. doi: 10.1073/pnas.52.5.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wiblin C. N., MacPherson I. A. The transformation of BHK 21 hamster cells by simian virus 40. Int J Cancer. 1972 Sep 15;10(2):296–309. doi: 10.1002/ijc.2910100210. [DOI] [PubMed] [Google Scholar]

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