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. 1974 Nov;71(11):4303–4306. doi: 10.1073/pnas.71.11.4303

Proton Magnetic Resonance Spectra of Rhodospirillum rubrum Cytochrome C2

Gary M Smith 1, Martin D Kamen 1
PMCID: PMC433870  PMID: 4373707

Abstract

Nuclear magnetic resonance spectra of ferro- and ferricytochrome C2 from the facultative photoheterotroph, Rhodospirillum rubrum, obtained with a 220-MHz spectrometer, are presented. Assignments to protons of various important structural groups in contact with or near the prosthetic heme group are given. These include (a) in the ferro-form, methyl, cβ and cγ protons of the extraplanar ligand residue, methionine 91, and methyl protons of the residue leucine 32; and (b) corresponding protons in the ferri-form with the addition of methyl protons from the condensation of a heme vinyl group with cysteine 17, a single proton from the condensation involving cysteine 14, and protons from the ring methyls. These resonances are compared with those corresponding to the same groups in horse heart cytochrome c.

Keywords: nuclear magnetic resonance assignments, cytochromes c, comparative structures

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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