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. 1974 Nov;71(11):4317–4321. doi: 10.1073/pnas.71.11.4317

lsomeric Phenylalanyl-tRNAs. Position of the Aminoacyl Moiety During Protein Biosynthesis

Sidney M Hecht 1,*, John W Kozarich 1, Francis J Schmidt 1
PMCID: PMC433873  PMID: 4612516

Abstract

The preparation of phenylalanyl-tRNA terminating in 3′-deoxyadenosine has been achieved by incubation of abbreviated tRNA (tRNA-CpCOH) with 3′-deoxyadenosine 5′-diphosphate and polynucleotide phosphorylase (EC 2.7.7.8), followed by aminoacylation. The isomeric phenylalanyl-tRNA terminating in 2′-deoxyadenosine was constructed by incubation of tRNA-CpCOH with 2′-deoxy-3′-O-phenylalanyladenosine 5′-diphosphate and polynucleotide phosphorylase. While tRNA is aminoacylated at the 2′-position, only the 3′-aminoacyl-tRNA is active as a peptide acceptor in the peptidyltransferase reaction. Both modified tRNAs were bound to the A-site as efficiently as unmodified tRNA, but neither was so efficient at P-site binding or as an acceptor in the peptidyltransferase reaction. Neither of the modified tRNAs acted as a donor in the peptidyltransferase reaction.

Keywords: CTP(ATP):tRNA nucleotidyltransferase, “chemical” tRNA aminoacylation, ribosomal binding assays, peptidyltransferase reaction, puromycin-like activity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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