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. 1974 Nov;71(11):4352–4355. doi: 10.1073/pnas.71.11.4352

A Pyruvate-Valine Enzyme Complex that is Dependent upon the Metabolic State of the Mitochondria

A Bergquist 1,2, E A Eakin 1,2, D K Murali 1,2, R P Wagner 1,2
PMCID: PMC433881  PMID: 4280069

Abstract

Data are presented suggesting that intact Neurospora crassa mitochondria contain an enzyme complex incorporating five enzymes necessary for the biosynthesis of isoleucine and valine. The functional integrity and stability of the complex has been shown to be dependent on the metabolic state of the mitochondria. The complex has been solubilized by digitonin and has an approximate molecular weight of 400,000.

Keywords: Neurospora crassa, isoleucine and valine synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altmiller D. H., Wagner R. P. Purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of Neurospora crassa. Arch Biochem Biophys. 1970 May;138(1):160–170. doi: 10.1016/0003-9861(70)90295-x. [DOI] [PubMed] [Google Scholar]
  2. Bergquist A., Eakin E. A., Eakin R. T., Wagner R. P. Growth, respiratory, and cytochrome characteristics of certain of the isoleucine-valine mutants of Neurospora crassa. Biochem Genet. 1974 Jul;12(1):39–49. doi: 10.1007/BF00487526. [DOI] [PubMed] [Google Scholar]
  3. Bergquist A., LaBrie D. A., Wagner R. P. Amino acid synthesis by the mitochondria of Neurospora crassa. I. Dependence on respiration of mitochondria. Arch Biochem Biophys. 1969 Nov;134(2):401–407. doi: 10.1016/0003-9861(69)90299-9. [DOI] [PubMed] [Google Scholar]
  4. Collins M., Wagner R. P. Branded chain amino acid aminotransferases of Neurospora crassa. Arch Biochem Biophys. 1973 Mar;155(1):184–193. doi: 10.1016/s0003-9861(73)80020-7. [DOI] [PubMed] [Google Scholar]
  5. Glatzer L., Eakin E., Wagner R. P. Acetohydroxy acid synthetase with a pH optimum of 7.5 from Neurospora crassa mitochondria: characterization and partial purification. J Bacteriol. 1972 Oct;112(1):453–464. doi: 10.1128/jb.112.1.453-464.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harding R. W., Caroline D. F., Wagner R. P. The pyruvate dehydrogenase complex from the mitochondrial fraction of Neurospora crassa. Arch Biochem Biophys. 1970 Jun;138(2):653–661. doi: 10.1016/0003-9861(70)90393-0. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Leiter E. H., LaBrie D. A., Bergquist A., Wagner R. P. In vitro mitochondrial complementation in Neurospora crassa. Biochem Genet. 1971 Dec;5(6):549–561. doi: 10.1007/BF00485673. [DOI] [PubMed] [Google Scholar]
  9. Marrink J., Gruber M. Molecular weight determination by chromatography on sepharose 4B. FEBS Lett. 1969 Feb;2(4):242–244. doi: 10.1016/0014-5793(69)80031-1. [DOI] [PubMed] [Google Scholar]
  10. WAGNER R. P., BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. doi: 10.1073/pnas.49.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]

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