Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Nov;71(11):4487–4490. doi: 10.1073/pnas.71.11.4487

Idiotypic Specificity of Surface Immunoglobulin and the Maturation of Leukemic Bone-marrow-derived Lymphocytes

S M Fu 1, R J Winchester 1, T Feizi 1,*, P D Walzer 1,†, H G Kunkel 1
PMCID: PMC433911  PMID: 4612522

Abstract

Certain cases of chronic lymphocytic leukemia are associated with monoclonal immunoglobulins in the serum. In the present study it was possible to demonstrate that the surface immunoglobulin of the leukemic lymphocytes was idiotypically identical to the serum monoclonal immunoglobulin of the same individual. This was done through the use of fluorescent antibodies that were prepared against the isolated immunoglobulin M kappa serum protein from patient Ei. Such antibodies, after absorption, failed to react with the lymphocytes of other individuals. Capping experiments indicated that both the immunoglobulin M and the immunoglobulin D on the leukemic cells possessed the same idiotypic specificity.

A population of plasma cells was also identified in the peripheral blood of patient Ei that showed strong intracellular staining with the idiotypic antiserum and was the apparent source of the specific serum immunoglobulin. The accumulated evidence indicates that the plasma cells were derived from the leukemic lymphocytes by a process of differentiation. Surface immunoglobulin D disappeared and surface immunoglobulin M decreased markedly in this process.

Keywords: chronic lymphocytic leukemia, differentiation

Full text

PDF
4487

Images in this article

4488Image
on p.4488

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AZAR H. A., HILL W. T., OSSERMAN E. F. Malignant lymphoma and lymphatic leukemia associated with myeloma-type serum proteins. Am J Med. 1957 Aug;23(2):239–249. doi: 10.1016/0002-9343(57)90197-3. [DOI] [PubMed] [Google Scholar]
  2. Fu S. M., Winchester R. J., Kunkel H. G. Occurrence of surface IgM, IgD, and free light chains of human lymphocytes. J Exp Med. 1974 Feb 1;139(2):451–456. doi: 10.1084/jem.139.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gamble C. N., Cutting H. O. The production of gamma G-globulin by lymphocytes in chronic lymphocytic leukemia. Immunocytologic investigation of a case. Blood. 1966 Feb;27(2):187–198. [PubMed] [Google Scholar]
  4. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  5. Knapp W., Bolhuis R. L., Rádl J., Hijmans W. Independent movement of IgD and IgM molecules on the surface of individual lymphocytes. J Immunol. 1973 Oct;111(4):1295–1298. [PubMed] [Google Scholar]
  6. Kubo R. T., Grey H. M., Pirofsky B. IgD: a major immunoglobulin on the surface of lymphocytes from patients with chronic lymphatic leukemia. J Immunol. 1974 May;112(5):1952–1954. [PubMed] [Google Scholar]
  7. Lindström F. D., Hardy W. R., Eberle B. J., Williams R. C., Jr Multiple myeloma and benign monoclonal gammopathy: differentiation by immunofluorescence of lymphocytes. Ann Intern Med. 1973 Jun;78(6):837–844. doi: 10.7326/0003-4819-78-6-837. [DOI] [PubMed] [Google Scholar]
  8. Pernis B., Forni L., Amante L. Immunoglobulin spots on the surface of rabbit lymphocytes. J Exp Med. 1970 Nov;132(5):1001–1018. doi: 10.1084/jem.132.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Preud'homme J. L., Seligmann M. Immunoglobulins on the surface of lymphoid cells in Waldenström's macroglobulinemia. J Clin Invest. 1972 Mar;51(3):701–705. doi: 10.1172/JCI106858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Seligmann M., Preud'Homme J. L., Brouet J. C. B and T cell markers in human proliferative blood diseases and primary immunodeficiencies, with special reference to membrane bound immunoglobulins. Transplant Rev. 1973;16:85–113. doi: 10.1111/j.1600-065x.1973.tb00118.x. [DOI] [PubMed] [Google Scholar]
  12. Shevach E. M., Stobo J. D., Green I. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J Immunol. 1972 May;108(5):1146–1151. [PubMed] [Google Scholar]
  13. Wernet P., Feizi T., Kunkel H. G. Idiotypic determinants of immunoglobulin M detected on the surface of human lymphocytes by cytotoxicity assays. J Exp Med. 1972 Sep 1;136(3):650–655. doi: 10.1084/jem.136.3.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zlotnick A., Robinson E. Chronic lymphatic leukemia associated with macroglobulinemia. Isr J Med Sci. 1970 May-Jun;6(3):365–372. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES