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. 1974 Nov;71(11):4556–4560. doi: 10.1073/pnas.71.11.4556

Utilization of Two Distinct Modes of Replication by a Hybrid Plasmid Constructed In Vitro from Separate Replicons

Kenneth Timmis 1, Felipe Cabello 1, Stanley N Cohen 1
PMCID: PMC433926  PMID: 4612523

Abstract

A hybrid plasmid, pSC134, that codes for two distinct sets of replication functions has been constructed in vitro by ligation of EcoRI endonuclease-cleaved pSC101 and Col E1 plasmid replicons, and has been introduced into Escherichia coli by transformation. The replication properties of the pSC134 plasmid in DNA polymerase I-defective mutants or in the presence of chloramphenicol indicate that this hybrid plasmid can utilize the functionally distinct modes of replication specified by both of its parent replicons.

Keywords: R-plasmids, DNA replication, restriction endonuclease, molecular cloning

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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