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. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579

Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA

Marjorie Thomas 1, John R Cameron 1, Ronald W Davis 1
PMCID: PMC433931  PMID: 4216019

Abstract

A bacteriophage λ strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the λ DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this λ strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.

Keywords: EcoRI restriction endonuclease, DNA joining, calcium transfection, electron microscopy

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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