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. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579

Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA

Marjorie Thomas 1, John R Cameron 1, Ronald W Davis 1
PMCID: PMC433931  PMID: 4216019

Abstract

A bacteriophage λ strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the λ DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this λ strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.

Keywords: EcoRI restriction endonuclease, DNA joining, calcium transfection, electron microscopy

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allet B., Jeppesen P. G., Katagiri K. J., Delius H. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI. Nature. 1973 Jan 12;241(5385):120–123. doi: 10.1038/241120a0. [DOI] [PubMed] [Google Scholar]
  2. Davis R. W., Parkinson J. S. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi. J Mol Biol. 1971 Mar 14;56(2):403–423. doi: 10.1016/0022-2836(71)90473-6. [DOI] [PubMed] [Google Scholar]
  3. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  4. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  5. Morrow J. F., Cohen S. N., Chang A. C., Boyer H. W., Goodman H. M., Helling R. B. Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1974 May;71(5):1743–1747. doi: 10.1073/pnas.71.5.1743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Murray N. E., De Ritis P. M., Foster L. A. DNA targets for the Escherichia coli K restriction system analysed genetically in recombinants between phages phi80 and lambda. Mol Gen Genet. 1973 Feb 2;120(3):261–281. doi: 10.1007/BF00267157. [DOI] [PubMed] [Google Scholar]
  7. Murray N. E., Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974 Oct 11;251(5475):476–481. doi: 10.1038/251476a0. [DOI] [PubMed] [Google Scholar]
  8. Parkinson J. S. Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics. 1968 Jul;59(3):311–325. doi: 10.1093/genetics/59.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]
  10. Rambach A., Tiollais P. Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927–3930. doi: 10.1073/pnas.71.10.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]

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