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. 1974 Dec;71(12):4658–4661. doi: 10.1073/pnas.71.12.4658

Latent DNA of Epstein-Barr Virus: Separation from High-Molecular-Weight Cell DNA in a Neutral Glycerol Gradient

Akiko Tanaka 1,2, Meihan Nonoyama 1,2
PMCID: PMC433954  PMID: 4373725

Abstract

Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm3 in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm3. The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.

Keywords: herpesvirus, latent viral genomes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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