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. 1974 Dec;71(12):4698–4702. doi: 10.1073/pnas.71.12.4698

Mutations That Alter the Transmission of Chloroplast Genes in Chlamydomonas

Ruth Sager 1, Zenta Ramanis 1
PMCID: PMC433963  PMID: 4531010

Abstract

Two mutations are described that alter the pattern of inheritance of chloroplast genes in Chlamydomonas. The mutant gene mat-1 linked to the mating type allele mt- greatly increases the frequency of exceptional zygotes, i.e., zygotes that transmit chloroplast genes from the mt- (male) parent. In some crosses, 80-90% of the zygotes are biparental, transmitting chloroplast genes from both parents. The mat-2 mutation, linked to mt+, acts to decrease the frequency of exceptional zygotes below the spontaneous level. The mutant effects are discussed in terms of a DNA modification-restriction system, postulated to regulate the transmission of chloroplast DNA in zygotes.

Keywords: maternal inheritance, cytoplasmic DNA, modification-restriction

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arber W., Linn S. DNA modification and restriction. Annu Rev Biochem. 1969;38:467–500. doi: 10.1146/annurev.bi.38.070169.002343. [DOI] [PubMed] [Google Scholar]
  2. Boyer H. W. DNA restriction and modification mechanisms in bacteria. Annu Rev Microbiol. 1971;25:153–176. doi: 10.1146/annurev.mi.25.100171.001101. [DOI] [PubMed] [Google Scholar]
  3. Cavalier-Smith T. Electron microscopic evidence for chloroplast fusion in zygotes of Chlamydomonas reinhardii. Nature. 1970 Oct 24;228(5269):333–335. doi: 10.1038/228333a0. [DOI] [PubMed] [Google Scholar]
  4. Chiang K. S. Physical conservation of parental cytoplasmic DNA through meiosis in Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1968 May;60(1):194–200. doi: 10.1073/pnas.60.1.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedmann I., Colwin A. L., Colwin L. H. Fine-structural aspects of fertilization in Chlamydomonas reinhardi. J Cell Sci. 1968 Mar;3(1):115–128. doi: 10.1242/jcs.3.1.115. [DOI] [PubMed] [Google Scholar]
  6. Meselson M., Yuan R., Heywood J. Restriction and modification of DNA. Annu Rev Biochem. 1972;41:447–466. doi: 10.1146/annurev.bi.41.070172.002311. [DOI] [PubMed] [Google Scholar]
  7. Sager R., Lane D. Molecular basis of maternal inheritance. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2410–2413. doi: 10.1073/pnas.69.9.2410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sager R. MENDELIAN AND NON-MENDELIAN INHERITANCE OF STREPTOMYCIN RESISTANCE IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1954 May;40(5):356–363. doi: 10.1073/pnas.40.5.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sager R., Ramanis Z. A genetic map of non-Mandelian genes in Chlamydomonas. Proc Natl Acad Sci U S A. 1970 Mar;65(3):593–600. doi: 10.1073/pnas.65.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sager R., Ramanis Z. Biparental inheritance of nonchromosomal genes induced by ultraviolet irradiation. Proc Natl Acad Sci U S A. 1967 Sep;58(3):931–937. doi: 10.1073/pnas.58.3.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schlanger G., Sager R. Localization of five antibiotic resistances at the subunit level in chloroplast ribosomes of Chlamydomonas. Proc Natl Acad Sci U S A. 1974 May;71(5):1715–1719. doi: 10.1073/pnas.71.5.1715. [DOI] [PMC free article] [PubMed] [Google Scholar]

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