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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Dec;71(12):4773–4777. doi: 10.1073/pnas.71.12.4773

Isolation of a Viral Polypeptide Associated with Poliovirus RNA Polymerase

R E Lundquist 1,*, E Ehrenfeld 1, J V Maizel Jr 1,*,†
PMCID: PMC433979  PMID: 4373729

Abstract

Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.

Keywords: picornavirus, replication complex, replicase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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