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. 1974 Dec;71(12):4783–4786. doi: 10.1073/pnas.71.12.4783

Hormonal Control of Messenger Ribonucleic Acid Metabolism in Barley Aleurone Layers

David Tuan-Hua Ho 1,2, J E Varner 1,2
PMCID: PMC433981  PMID: 4548189

Abstract

Ribonucleic acid containing poly(adenylic acid) [poly(A)-RNA] is present in barley aleurone layers. This poly(A)-RNA becomes labeled with radioactive precursors of RNA during the incubation of isolated aleurone layers with or without gibberellic acid. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within 3-4 hr of addition of the hormone and reaches a maximum, which is about 50-60% over the control, 10-12 hr after addition of the hormone. Cordycepin inhibits total RNA as well as poly(A)-RNA synthesis in barley aleurone layers. However, cordycepin inhibits the hormone-controlled synthesis of α-amylase (EC 3.2.1.1) only if it is added 12 hr or less after gibberellic acid. The insensitivity of α-amylase production to cordycepin after 12 hr of gibberellic acid treatment suggests that α-amylase is translated from stable messenger RNA.

Keywords: gibberellic acid, poly(A)-RNA, cordycepin, stable mRNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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