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SDS–PAGE analysis of purified, recombinant hexosaminidases. Purified recombinant enzymes were either incubated with water (−) or N-glycosidase F (+) and subjected to SDS–PAGE and staining with Coomassie Brilliant Blue. Dagger indicates the enzyme produced in P. pastoris X-33 and asterisk indicates the enzyme produced in Hi5 insect cells. Band at approximately 30 kDa in (+) lanes corresponds to the N-glycosidase F protein. All C. elegans enzymes were produced in P. pastoris X-33.
