Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Dec;71(12):4897–4900. doi: 10.1073/pnas.71.12.4897

An Electron-Nuclear Double Resonance (Endor) Study of the Special Pair Model for Photo-Reactive Chlorophyll in Photosynthesis

J R Norris 1, H Scheer 1, M E Druyan 1, J J Katz 1
PMCID: PMC434006  PMID: 16592206

Abstract

A comparison of hyperfine coupling constants obtained by electron-nuclear double resonance spectroscopy of in vitro monomer chlorophyll and bacteriochlorophyll free radicals with those of the photoesr (electron spin resonance) signal associated with light conversion in photosynthesis provides convincing support for the special pair model for the in vivo photo-reaction center.

Keywords: hyperfine coupling constants, photo electron spin resonance, photosynthetic bacteria, selective deuteration

Full text

PDF
4897

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Androes G. M., Singleton M. F., Calvin M. EPR IN CHROMATOPHORES FROM RHODOSPIRILLUM RUBRUM AND IN QUANTASOMES FROM SPINACH CHLOROPLASTS. Proc Natl Acad Sci U S A. 1962 Jun;48(6):1022–1031. doi: 10.1073/pnas.48.6.1022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ballschmiter K., Truesdell K., Katz J. J. Aggregation of chlorophyll in nonpolar solvents from molecular weight measurements. Biochim Biophys Acta. 1969 Sep 2;184(3):604–613. doi: 10.1016/0304-4165(69)90275-x. [DOI] [PubMed] [Google Scholar]
  3. Bolton J. R., Clayton R. K., Reed D. W. An identification of the radical giving rise to the light-induced electron spin resonance signal in photosynthetic bacteria. Photochem Photobiol. 1969 Mar;9(3):209–218. doi: 10.1111/j.1751-1097.1969.tb07285.x. [DOI] [PubMed] [Google Scholar]
  4. Borg D. C., Fajer J., Felton R. H., Dolphin D. The pi-Cation Radical of Chlorophyll a. Proc Natl Acad Sci U S A. 1970 Oct;67(2):813–820. doi: 10.1073/pnas.67.2.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Calvin M., Sogo P. B. Primary Quantum Conversion Process in Photosynthesis: Electron Spin Resonance. Science. 1957 Mar 15;125(3246):499–500. doi: 10.1126/science.125.3246.499. [DOI] [PubMed] [Google Scholar]
  6. Commoner B., Heise J. J., Townsend J. LIGHT-INDUCED PARAMAGNETISM IN CHLOROPLASTS. Proc Natl Acad Sci U S A. 1956 Oct;42(10):710–718. doi: 10.1073/pnas.42.10.710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Druyan M. E., Norris J. R., Katz J. J. Electron spin resonance of (25 Mg)chlorophyll a. J Am Chem Soc. 1973 Mar 7;95(5):1682–1683. doi: 10.1021/ja00786a067. [DOI] [PubMed] [Google Scholar]
  8. Fajer J., Borg D. C., Forman A., Dolphin D., Felton R. H. pi-Cation radicals and dications of metalloporphyrins. J Am Chem Soc. 1970 Jun 3;92(11):3451–3459. doi: 10.1021/ja00714a038. [DOI] [PubMed] [Google Scholar]
  9. Fong F. K. Molecular basis for the photosynthetic primary process. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3692–3695. doi: 10.1073/pnas.71.9.3692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fuhrhop J. H., Mauzerall D. The one-electron oxidation of metalloporphyrins. J Am Chem Soc. 1969 Jul 16;91(15):4174–4181. doi: 10.1021/ja01043a027. [DOI] [PubMed] [Google Scholar]
  11. Katz J. J., Ballschmiter K., Garcia-Morin M., Strain H. H., Uphaus R. A. Electron paramagnetic resonance of chlorophyll-water aggregates. Proc Natl Acad Sci U S A. 1968 May;60(1):100–107. doi: 10.1073/pnas.60.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katz J. J. Chlorophyll interactions and light conversion in photosynthesis. Naturwissenschaften. 1973 Jan;60(1):32–39. doi: 10.1007/BF00591761. [DOI] [PubMed] [Google Scholar]
  13. Kohl D. H., Townsend J., Commoner B., Crespi H. L., Dougherty R. C., Katz J. J. Effects of isotopic substitution on electron spin resonance signals in photosynthetic organisms. Nature. 1965 Jun 12;206(989):1105–1110. doi: 10.1038/2061105a0. [DOI] [PubMed] [Google Scholar]
  14. Kok B. Light Induced Absorption Changes in Photosynthetic Organisms. II. A Split-beam Difference Spectrophotometer. Plant Physiol. 1959 May;34(3):184–192. doi: 10.1104/pp.34.3.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Loach P. A., Hadsell R. M., Sekura D. L., Stemer A. Quantitative dissolution of the membrane and preparation of photoreceptor subunits from Rhodospirillum rubrum. Biochemistry. 1970 Aug 4;9(16):3127–3135. doi: 10.1021/bi00818a003. [DOI] [PubMed] [Google Scholar]
  16. McElroy J. D., Feher G., Mauzerall D. C. Characterization of primary reactants in bacterial photosynthesis. I. Comparison of the light-induced EPR signal (g=2.0026) with that of a bacteriochlorophyll radical. Biochim Biophys Acta. 1972 May 25;267(2):363–374. doi: 10.1016/0005-2728(72)90123-5. [DOI] [PubMed] [Google Scholar]
  17. McElroy J. D., Feher G., Mauzerall D. C. On the nature of the free radical formed during the primary process of bacterial photosynthesis. Biochim Biophys Acta. 1969 Jan 14;172(1):180–183. doi: 10.1016/0005-2728(69)90105-4. [DOI] [PubMed] [Google Scholar]
  18. Norris J. R., Druyan M. E., Katz J. J. Electron nuclear double resonance of bacteriochlorophyll free radical in vitro and in vivo. J Am Chem Soc. 1973 Mar 7;95(5):1680–1682. doi: 10.1021/ja00786a066. [DOI] [PubMed] [Google Scholar]
  19. Norris J. R., Uphaus R. A., Crespi H. L., Katz J. J. Electron spin resonance of chlorophyll and the origin of signal I in photosynthesis. Proc Natl Acad Sci U S A. 1971 Mar;68(3):625–628. doi: 10.1073/pnas.68.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Norris J. R., Uphaus R. A., Katz J. J. Electron spin resonance in 13 C-labelled chlorophyll and 13 C-labelled algae. Biochim Biophys Acta. 1972 Aug 17;275(2):161–168. doi: 10.1016/0005-2728(72)90036-9. [DOI] [PubMed] [Google Scholar]
  21. Otten H. A. Absorption changes in the reaction center of photosynthetic bacteria and - electron calculations on bacteriochlorophyll, its mono cation and anion. Photochem Photobiol. 1971 Nov;14(5):589–596. doi: 10.1111/j.1751-1097.1971.tb06198.x. [DOI] [PubMed] [Google Scholar]
  22. Warden J. T., Jr, Bolton J. R. Simultaneous quantitative comparison of the optical changes at 700 nm (p700) and electron spin resonance signals in system I of green plant photosynthesis. J Am Chem Soc. 1973 Sep 19;95(19):6435–6436. doi: 10.1021/ja00800a046. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES