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. 1974 Dec;71(12):4965–4969. doi: 10.1073/pnas.71.12.4965

Endonuclease Activity Associated with Purified PM2 Bacteriophages

Françoise Laval 1,*
PMCID: PMC434020  PMID: 4531030

Abstract

An endonucleolytic activity is associated with purified PM2 bacteriophages. It converts the double-stranded supercoiled PM2 DNA mostly into the linear form that sediments as a homogenous peak in alkaline sucrose gradients. The same activity is found in intact or detergent-lysed phages. Optimal activity is observed between pH 6.8 and 7.5 at 28°. Divalent cations, Mg2+ or Mn2+, are necessary for activity, and the enzyme is inhibited by RNA. The endonuclease has no appreciable activity on linear DNA molecules, but it attacks single-stranded circular DNA.

Keywords: DNase; zone sedimentation; electron microscopy; PM2, simian virus 40, and fd supercoiled DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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