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. 1974 Dec;71(12):4991–4994. doi: 10.1073/pnas.71.12.4991

Studies on the Reverse Transcriptase of RNA Tumor Viruses. Structural Relatedness of Two Subunits of Avian RNA Tumor Viruses*

Wade Gibson 1, Inder M Verma 1
PMCID: PMC434025  PMID: 4140514

Abstract

The structural relationship of the small (α) and large (β) subunits of reverse transcriptase isolated from two avian RNA tumor viruses has been examined by tryptic peptide analysis. Comparison of the tryptic hydrolysates of the isolated subunits by two-dimensional separation on thin-layer cellulose plates indicates that (i) the α subunit of reverse transcriptase of avian myeloblastosis virus is structurally related to the β subunit; (ii) the α and β subunits of the enzyme of Rous sarcoma virus also appear to be related; and (iii) there appears to be an extensive amino-acid sequence homology between reverse transcriptases of avian myeloblastosis virus and Rous sarcoma virus. Evidence is also presented that both α and β subunits can be identified in purified avian myeloblastosis virions.

Keywords: polyacrylamide gel electrophoresis, in vitro radiolabeling with 125I, two-dimensional peptide analysis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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