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. 1974 Dec;71(12):5017–5021. doi: 10.1073/pnas.71.12.5017

Deoxysubstitution in RNA by RNA Polymerase In Vitro: A New Approach to Nucleotide Sequence Determinations

Gary V Paddock 1, Howard C Heindell 1, Winston Salser 1
PMCID: PMC434031  PMID: 4612537

Abstract

Deoxynucleotides have been incorporated into RNA synthesized in vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.

Keywords: pancreatic DNase I, U1 ribonuclease, U2 ribonuclease, pancreatic ribonuclease A, ribosubstituted DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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