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. 1974 Dec;71(12):5057–5061. doi: 10.1073/pnas.71.12.5057

Nonhistone Proteins Control Gene Expression in Reconstituted Chromatin

T Barrett 1, D Maryanka 1, P H Hamlyn 1, H J Gould 1
PMCID: PMC434039  PMID: 4140516

Abstract

Chromatin was reconstituted from the purified DNA and histones of chicken erythrocytes and the nonhistone proteins of either chicken reticulocytes or chicken liver. Reconstituted chromatins, native chicken reticulocyte chromatin, and free DNA were transcribed with Escherichia coli RNA polymerase and the concentrations of globin-specific sequences in the RNA products were measured by hybridization with [3H]DNA complementary to chicken globin messenger RNA. Reticulocyte, but not liver, nonhistone proteins were shown to activate the globin genes in reconstituted erythrocyte chromatin. The transcripts of native and reconstituted chromatins were indistinguishable in respect of both the total yield of the RNA and the fractional yield of globin-specific sequences.

Keywords: nonhistones, histones, chicken globin mRNA, complementary DNA, RNA polymerase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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