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. 1974 Oct;71(10):3854–3857. doi: 10.1073/pnas.71.10.3854

Relaxation Complexes of Plasmids ColE1 and ColE2: Unique Site of the Nick in the Open Circular DNA of the Relaxed Complexes

Michael A Lovett 1, Donald G Guiney 1, Donald R Helinski 1
PMCID: PMC434282  PMID: 4610563

Abstract

The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with the EcoRI restriction endonuclease demonstrates that the single-strand break is at a unique position. The site of the single-strand break in the relaxed ColE1 complex is approximately the same distance from the EcoRI cleavage site as the origin of ColE1 DNA replication.

Keywords: Escherichia coli, supercoiled DNA, endonuclease, restriction enzyme, DNA replication

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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