Abstract
A derivative of λ b221 that has lost by mutation all EcoRI restriction sites has been isolated by alternative growth on restrictive and nonrestrictive strains. It has an efficiency of plating equal to 1 on the restrictive strain. Genetic cross of this bacteriophage with λ plac5 imm21 gave rise to recombinants of intermediate restricting ratios. The analysis of the EcoRI endonuclease-cleaved DNA by polyacrylamide gel electrophoresis, compared with the genetic results, has permitted identification of EcoRI endonuclease cleavage sites in the recombinants. The genotypes are: λ plac5 CI857 sRIλ30sRIλ20sRIλ10 and λ plac5 CI857 sRIλ20sRIλ10. The remaining cleavage sites, respectively, sRIlac sRIλ4 and sRIlac sRIλ4 sRIλ3, are all located in a region nonessential for bacteriophage multiplication. The involvement of these mutant bacteriophages as vector for foreign genes are discussed.
Keywords: restriction-resistant mutant, genetic cross, gel electrophoresis
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allet B., Jeppesen P. G., Katagiri K. J., Delius H. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI. Nature. 1973 Jan 12;241(5385):120–123. doi: 10.1038/241120a0. [DOI] [PubMed] [Google Scholar]
- Arber W., Kühnlein U. Mutationeller Verlust B-spezifischer Restriktion des Bakteriophagen fd. Pathol Microbiol (Basel) 1967;30(6):946–952. [PubMed] [Google Scholar]
- Arber W., Linn S. DNA modification and restriction. Annu Rev Biochem. 1969;38:467–500. doi: 10.1146/annurev.bi.38.070169.002343. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beckwith J. R., Signer E. R. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80. J Mol Biol. 1966 Aug;19(2):254–265. doi: 10.1016/s0022-2836(66)80003-7. [DOI] [PubMed] [Google Scholar]
- Chang A. C., Cohen S. N. Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030–1034. doi: 10.1073/pnas.71.4.1030. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. N., Chang A. C., Boyer H. W., Helling R. B. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240–3244. doi: 10.1073/pnas.70.11.3240. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eskin B., Lautenberger J. A., Linn S. Host-controlled modification and restriction of bacteriophage T7 by escherichia coli B. J Virol. 1973 Jun;11(6):1020–1023. doi: 10.1128/jvi.11.6.1020-1023.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson D. A., Symons R. H., Berg P. Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904–2909. doi: 10.1073/pnas.69.10.2904. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse M L, Lederberg E M, Lederberg J. Transduction in Escherichia Coli K-12. Genetics. 1956 Jan;41(1):142–156. doi: 10.1093/genetics/41.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]