Abstract
A mutant resistant to methanol was isolated and the mutation was shown to be located on the same linkage group with two other previously identified markers, white spore color and temperature sensitivity. In the course of analyzing methanol-resistant haploid segregants, four classes of methanol-resistant diploids were found. It is proposed that these represent mitotic recombinants, and a map of the chromosome is suggested. Segregation analysis of the diploids shows the genotypes of the diploids to be in perfect agreement with the mitotic recombination model.
Keywords: parasexual genetics, linkage, genetic map, drug-resistant mutants
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Katz E. R., Sussman M. Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants (clones-temperature sensitive-ploidy-fruiting bodies-spore-slime mold). Proc Natl Acad Sci U S A. 1972 Feb;69(2):495–498. doi: 10.1073/pnas.69.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PONTECORVO G., KAFER E. Genetic analysis based on mitotic recombination. Adv Genet. 1958;9:71–104. [PubMed] [Google Scholar]
- ROMAN H. Studies of gene mutation in Saccharomyces. Cold Spring Harb Symp Quant Biol. 1956;21:175–185. doi: 10.1101/sqb.1956.021.01.015. [DOI] [PubMed] [Google Scholar]
- Stern C. Somatic Crossing over and Segregation in Drosophila Melanogaster. Genetics. 1936 Nov;21(6):625–730. doi: 10.1093/genetics/21.6.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams K. L., Kessin R. H., Newell P. C. Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. J Gen Microbiol. 1974 Sep;84(1):59–69. doi: 10.1099/00221287-84-1-59. [DOI] [PubMed] [Google Scholar]