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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Oct;71(10):4077–4081. doi: 10.1073/pnas.71.10.4077

Initiation of Oncogenic Transformation of Mouse Lymphocytes In Vitro by Abelson Leukemia Virus

Marshall D Sklar *,, Beverly J White , Wallace P Rowe *
PMCID: PMC434331  PMID: 4372605

Abstract

While C-type RNA viruses are known to induce leukemias and lymphomas, oncogenic transformation of lymphoid cells by them in vitro has not been reported. In this study, splenocytes from female BALB/c mice were infected in vitro with Abelson virus, a C-type RNA virus that induces nonthymic lymphomas and plasmacytomas in mice. The cells were transplanted into recipients of different karyotype, either male BALB/c mice or hybrid BALB/c × AL/N (CALF1) mice, which bear the Rb(5.19)1Wh translocation. Transplants of eight of the resulting tumors (one plasmacytoma and seven lymphomas) contained cells of donor BALB/c karyotype, indicating that transformation of splenocytes occurred in vitro.

Keywords: murine lymphoma, B-lymphocytes, plasmacytoma, karyologic markers, BALB/c mice

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Selected References

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  1. Abelson H. T., Rabstein L. S. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 1970 Aug;30(8):2213–2222. [PubMed] [Google Scholar]
  2. Caspersson T., Zech L., Modest E. J. Fluorescent labeling of chromosomal DNA: superiority of quinacrine mustard to quinacrine. Science. 1970 Nov 13;170(3959):762–762. doi: 10.1126/science.170.3959.762. [DOI] [PubMed] [Google Scholar]
  3. Click R. E., Benck L., Alter B. J. Immune responses in vitro. I. Culture conditions for antibody synthesis. Cell Immunol. 1972 Feb;3(2):264–276. doi: 10.1016/0008-8749(72)90165-7. [DOI] [PubMed] [Google Scholar]
  4. Hulett H. R., Bonner W. A., Sweet R. G., Herzenberg L. A. Development and application of a rapid cell sorter. Clin Chem. 1973 Aug;19(8):813–816. [PubMed] [Google Scholar]
  5. Julius M. H., Masuda T., Herzenberg L. A. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1934–1938. doi: 10.1073/pnas.69.7.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lieberman M., Kaplan H. S. Lymphoid tumor induction by mouse thymocytes infected in vitro with radiation leukemia virus. Natl Cancer Inst Monogr. 1966 Sep;22:549–557. [PubMed] [Google Scholar]
  7. Miller O. J., Miller D. A., Kouri R. E., Allderdice P. W., Dev V. G., Grewal M. S., Hutton J. J. Identification of the mouse karyotype by quinacrine fluorescence, and tentative assignment of seven linkage groups. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1530–1533. doi: 10.1073/pnas.68.7.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Potter M., Pumphrey J. G., Walters J. L. Growth of primary plasmacytomas in the mineral oil-conditioned peritoneal environment. J Natl Cancer Inst. 1972 Jul;49(1):305–308. [PubMed] [Google Scholar]
  9. Potter M., Sklar M. D., Rowe W. P. Rapid viral induction of plasmacytomas in pristane-primed BALB-c mice. Science. 1973 Nov 9;182(4112):592–594. doi: 10.1126/science.182.4112.592. [DOI] [PubMed] [Google Scholar]
  10. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  11. Watson J., Epstein R., Nakoinz I., Ralph P. The role of humoral factors in the initiation of in vitro primary immune responses. II. Effects of lymphocyte mitogens. J Immunol. 1973 Jan;110(1):43–52. [PubMed] [Google Scholar]
  12. White B. J., Tjio J. H., Van de Water L. C., Crandall C. Trisomy for the smallest autosome of the mouse and identification of the T1Wh translocation chromosome. Cytogenetics. 1972;11(5):363–378. doi: 10.1159/000130203. [DOI] [PubMed] [Google Scholar]

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