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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Oct;71(10):4159–4163. doi: 10.1073/pnas.71.10.4159

Detection of Mutagenic Activity in Cigarette Smoke Condensates

Larry D Kier 1, Edith Yamasaki 1, Bruce N Ames 1
PMCID: PMC434349  PMID: 4610572

Abstract

The Salmonella typhimurium microsomal test system for mutagenic activity was successfully used to detect the presence of mutagenic compounds in the smoke condensates of several types of cigarettes. The condensates were shown to contain compounds which could cause frameshift mutations when activated by microsomal enzymes. An analysis of fractions of smoke condensate revealed that the detected mutagenic activity was distributed in several of the fractions. Most of the activity of the whole condensate was in basic fractions and in a weakly acidic fraction. Condensates from cigarettes treated with magnesium nitrate differed from other condensates in two respects. They contained frameshift mutagens which did not require microsomal activation and mutagens which could cause base-pair substitution mutations. Although the detection system usually employs rat liver microsomal preparations, a rat lung microsomal preparation was also found to be capable of converting smoke condensates and known chemical carcinogens into mutagenic forms.

Keywords: carcinogenesis, Salmonella tester strains, microsomal activation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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