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. 1974 Oct;71(10):4183–4187. doi: 10.1073/pnas.71.10.4183

Viral DNA in Transformed Cells. III. The Amounts of Different Regions of the SV40 Genome Present in a Line of Transformed Mouse Cells*

Michael Botchan 1, Brad Ozanne 1, Bill Sugden 1, Phillip A Sharp 1, Joe Sambrook 1
PMCID: PMC434354  PMID: 4372611

Abstract

32P-Labeled SV40 DNA was treated sequentially with restricting endonucleases EcoRI and Hpa I, and the resulting four fragments of DNA were separated by gel electrophoresis. The kinetics of renaturation of each of the fragments and of complete SV40 DNA were measured in the presence of DNA extracted from the SVT2 line of SV40-transformed mouse cells. It was found that these cells contain about six copies of a segment of DNA which includes the early region of the SV40 genome, and about one copy of the late viral sequences.

To map the region of the viral genome which is transcribed in SVT2 cells, separated strands of each of the four fragments were prepared and hybridized to total transformed cell RNA. Part of the E strands of the two DNA fragments (A and C) which span the early region of the SV40 genome were found to enter the hybrid.

Keywords: restriction enzymes, C0t curves, transcription

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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