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. 1974 Oct;71(10):4213–4216. doi: 10.1073/pnas.71.10.4213

On Reconstitution of Bacterial Photophosphorylation In Vitro

Augusto F Garcia 1,*, Gerhart Drews 1,†, Martin D Kamen 1,‡
PMCID: PMC434360  PMID: 4530296

Abstract

Membranes active in oxidative phosphorylation but inactive in photophosphorylation were isolated from a carotenoid-less and bacteriochlorophyll-less mutant strain of Rhodopseudomonas capsulata. These membranes were uncoupled and recombined with coupling factor from the same strain and with reaction centers from a photosynthetically active revertant strain. The reconstituted system was able to form ATP from ADP and inorganic phosphate under anaerobic conditions in the light. Rates of 2 nmoles of ATP formed per min per μg of bacteriochlorophyll were obtained. A high incorporation of 32P from inorganic phosphate into ADP in the light as well as in the dark was observed, the activity of which was associated with coupling factor. This incorporation occurs both aerobically and anaerobically and appears to be independent of photophosphorylation.

Keywords: reaction center, coupling factor, phosphorylating inactive membrane, Rhodopseudomonas capsulata

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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