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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Oct;71(10):4241–4245. doi: 10.1073/pnas.71.10.4241

Reciprocal Inhibition of Mouse Leukemia Virus Infection by Fv-1 Allele Cell Extracts

Raymond W Tennant 1, Bonnie Schluter *, Wen-Kuang Yang 1, Arthur Brown *
PMCID: PMC434367  PMID: 4372614

Abstract

Soluble extracts of mouse cells with Fv-1n or Fv-1b gene alleles specifically and reciprocally inhibit infection of B- or N-tropic mouse leukemia viruses in permissive cell cultures. NB-tropic virus infection was not inhibited by either cell extract. Extracts from Fv-1- cells did not inhibit infection by the three virus host-range types, but N- or B-tropic virus infection of Fv-1- cells was inhibited by extracts of the nonpermissive cells, and Fv-1nb cell extracts inhibited both viruses. The maximum degree of inhibition was 50-80% as determined by immunofluorescent or plaque assays, with extracts containing up to 500 μg/ml of nonpermissive cell protein. The inhibitor(s) is relatively unstable since activity is lost after 2 hr at 37° or 30 min at 56°. The inhibitor(s) was most effective if added 2 hr before or within 2 hr after infection, did not react with the virus directly, inhibit virus attachment, or inhibit the normal cell functions tested. These results indicate that nonpermissive mouse cells contain a product, possibly determined by the Fv-1 gene, which inhibits some early postpenetration event(s) in leukemia virus infection.

Keywords: gene action, cell culture, virus host range, restriction mechanism

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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