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. 1994 Apr 12;91(8):2905–2908. doi: 10.1073/pnas.91.8.2905

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.

C L Peterson 1, A Dingwall 1, M P Scott 1
PMCID: PMC43482  PMID: 8159677

Abstract

The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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