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. 1994 Apr 12;91(8):2960–2964. doi: 10.1073/pnas.91.8.2960

Nervous control of juvenile hormone biosynthesis in Locusta migratoria.

G Horseman 1, R Hartmann 1, M Virant-Doberlet 1, W Loher 1, F Huber 1
PMCID: PMC43494  PMID: 8159687

Abstract

In Locusta migratoria migratorioides R. and F., two types of brain neurons innervate the juvenile hormone (JH)-producing corpora allata (CA). Thirteen cells in each pars lateralis (PL) innervate the ipsilateral CA, while four cells (two in each PL) innervate both glands. We investigated possible influences of these two neuronal types on JH production by a newly developed method. A radiochemical assay was used to measure hourly JH production by a CA with intact nerve connections to the brain. Then, changes in hormone production due to selective nerve stimulation or transection were assessed. In control preparations JH production per h remained approximately constant for at least 9 h. Simultaneous electrical stimulation of all neurons innervating one CA (i.e., 13 ipsilateral plus 4 bilaterally innervating cells) always inhibited JH production, while their transection led to a rapid progressive increase in JH biosynthesis in CA from females with oocytes longer than 4.5 mm. Thus, there is strong neurally mediated inhibition of the CA at certain phases of the vitellogenic cycle. The dramatic effects of nerve transection show that in vitro rates of JH production are an unreliable indicator of in vivo levels. Selective stimulation of the four neurons innervating both CA suggests that they do modulate JH biosynthesis but the effect varies qualitatively depending on the phase of the vitellogenic cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Copenhaver P. F., Truman J. W. Metamorphosis of the cerebral neuroendocrine system in the moth Manduca sexta. J Comp Neurol. 1986 Jul 8;249(2):186–204. doi: 10.1002/cne.902490206. [DOI] [PubMed] [Google Scholar]
  2. Gray R. S., Muehleisen D. P., Katahira E. J., Bollenbacher W. E. A 28-kDa cerebral neuropeptide from Manduca sexta: relationship to the insect prothoracicotropic hormone. Cell Mol Neurobiol. 1993 Feb;13(1):39–58. doi: 10.1007/BF00712988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kataoka H., Toschi A., Li J. P., Carney R. L., Schooley D. A., Kramer S. J. Identification of an allatotropin from adult manduca sexta. Science. 1989 Mar 17;243(4897):1481–1483. doi: 10.1126/science.243.4897.1481. [DOI] [PubMed] [Google Scholar]
  4. Kawakami A., Kataoka H., Oka T., Mizoguchi A., Kimura-Kawakami M., Adachi T., Iwami M., Nagasawa H., Suzuki A., Ishizaki H. Molecular cloning of the Bombyx mori prothoracicotropic hormone. Science. 1990 Mar 16;247(4948):1333–1335. doi: 10.1126/science.2315701. [DOI] [PubMed] [Google Scholar]
  5. Khan M. A., Koopmanschap A. B., de Kort C. A. The relative importance of nervous and humoral pathways for control of corpus allatum activity in the adult Colorado potato beetle, Leptinotarsa decemlineata (Say). Gen Comp Endocrinol. 1983 Nov;52(2):214–221. doi: 10.1016/0016-6480(83)90115-6. [DOI] [PubMed] [Google Scholar]
  6. Kramer S. J., Toschi A., Miller C. A., Kataoka H., Quistad G. B., Li J. P., Carney R. L., Schooley D. A. Identification of an allatostatin from the tobacco hornworm Manduca sexta. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9458–9462. doi: 10.1073/pnas.88.21.9458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. O'Brien M. A., Katahira E. J., Flanagan T. R., Arnold L. W., Haughton G., Bollenbacher W. E. A monoclonal antibody to the insect prothoracicotropic hormone. J Neurosci. 1988 Sep;8(9):3247–3257. doi: 10.1523/JNEUROSCI.08-09-03247.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pratt G. E., Farnsworth D. E., Feyereisen R. Changes in the sensitivity of adult cockroach corpora allata to a brain allatostatin. Mol Cell Endocrinol. 1990 Apr 17;70(2):185–195. doi: 10.1016/0303-7207(90)90158-5. [DOI] [PubMed] [Google Scholar]
  9. Pratt G. E., Tobe S. S. Juvenile hormones radiobiosynthesised by corpora allata of adult female locusts in vitro. Life Sci. 1974 Feb 1;14(3):575–586. doi: 10.1016/0024-3205(74)90372-5. [DOI] [PubMed] [Google Scholar]
  10. Stay B., Chan K. K., Woodhead A. P. Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata. Cell Tissue Res. 1992 Oct;270(1):15–23. doi: 10.1007/BF00381875. [DOI] [PubMed] [Google Scholar]
  11. Stay B., Tobe S. S. Control of juvenile hormone biosynthesis during the reproductive cycle of a viviparous cockroach. I. Activation and inhibition of corpora allata. Gen Comp Endocrinol. 1977 Dec;33(4):531–540. doi: 10.1016/0016-6480(77)90113-7. [DOI] [PubMed] [Google Scholar]
  12. Tobe S. S., Pratt G. E. The influence of substrate concentrations on the rate of insect juvenile hormone biosynthesis by corpora allata of the desert locust in vitro. Biochem J. 1974 Oct;144(1):107–113. doi: 10.1042/bj1440107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Westbrook A. L., Regan S. A., Bollenbacher W. E. Developmental expression of the prothoracicotropic hormone in the CNS of the tobacco hornworm Manduca sexta. J Comp Neurol. 1993 Jan 1;327(1):1–16. doi: 10.1002/cne.903270102. [DOI] [PubMed] [Google Scholar]
  14. Woodhead A. P., Stay B., Seidel S. L., Khan M. A., Tobe S. S. Primary structure of four allatostatins: neuropeptide inhibitors of juvenile hormone synthesis. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5997–6001. doi: 10.1073/pnas.86.15.5997. [DOI] [PMC free article] [PubMed] [Google Scholar]

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