Figure 9. IL-6 release by macrophages (A–D) and microglial cells (E–H) from young and aged mice in vitro.

In non-activated state, IL-6 release form aged and young macrophages did not differ (n = 14/21) (A). IL-6 concentrations in supernatants of aged and young macrophages were below the detection limit (E). Macrophages from aged mice released less IL-6 than macrophages from young mice after treatment with different concentrations of Pam₃CSK₄ (n = 12–18) (B), LPS (n = 6–15) (C), and CpG (n = 9–15) (D). Similarly, microglial cells from aged mice released less IL-6 than microglial cells from young mice after treatment with a selected dose of Pam₃CSK₄ (n = 6) (F), LPS (n = 6) (G), and CpG (n = 5) (H). Data are shown as medians (25./75. percentile); *p < 0.05, **p < 0.01, ***p < 0.001, Mann-Whitney U-test, Bonferroni correction in B, C, and D.