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. 2014 Dec 10;5(24):12555–12572. doi: 10.18632/oncotarget.2717

Figure 10. Phosphorylation of Tip60 at T158 by p38α is required for p53-K120 acetylation and PUMA induction after DNA damage.

Figure 10

(A–B) U2OS cells were co-transduced with shRNA for GFP (shGFP) or Tip60 (shTip60-887 or -1506) and vector (WN), wild type mouse Tip60 (WT) or mutant mouse Tip60 (T158A). Cells were treated with 1 μM of Dox for 36 h (A) or 10 Gy of γ-radiation followed by incubation for 24 h (B). Cell lysis was subjected to Western blot analysis detecting the indicated proteins. (C–D) U2OS cells were co-transduced with shRNA for GFP (shGFP) or Tip60 (shTip60-887 or -1506) and vector (WN), wild type mouse Tip60 (WT) or mutant mouse Tip60 (T158A). Cells were treated with 1 μM of Dox for 36 h (C) or 10 Gy of γ-radiation followed by incubation for 24 h (D) mRNA levels of PUMA (right panels) and p21WAF1 (left panels) were detected by real-time PCR. Values are mean ± SEM for triplicates.