Fig. 4.

Nanostring data for effect of MASOs on regulatory mRNA levels. Simultaneous quantitative assessment of transcript levels for 181 regulatory genes and signaling ligands in control embryos and in those bearing MASOs as indicated. Control counts (abscissa) are plotted against experimental counts (ordinate); values shown are counted molecules of each mRNA species per sample. Each sample contained RNA extracted from 33 embryos. Red envelopes indicate the maximum range of scatter as observed in large numbers of control runs with this instrument; from moderate levels of abundance onwards the limit of this variation is approximately twofold. Genes that fall outside these limits are considered to be significantly affected by the MASO treatment. These genes are represented by red dots; those in black text are relevant to the subject of this work, while others are shown in gray. The vast majority of the regulatory genes assessed are expressed the same as in the control in all experiments, and are represented as black dots within the red significance envelope. (Aa,b) Effects of soxb1 MASO assessed prior to and after CB formation at 18 and 35 hpf, respectively. (B-D) Effects of onecut (B), otxβ1/2 (C) and foxg (D) MASOs.