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PROTEIN SCIENCE A PUBLICATION OF THE PROTEIN SOCIETY

EDITOR-IN-CHIEF

Brian W. Matthews University of Oregon

FOUNDING EDITOR

Hans Neurath

ASSOCIATE EDITORS

Hideo Akutsu Osaka University

Dorothy Beckett University of Maryland

Markus Gruetter University of Zurich

Ron Levy Temple University

Albert J.R. Heck Utrecht Institute for Pharmaceutical Sciences trecht University

Dr. Yigong Shi School of Life Science Tsinghua University

INTERACTIVE IMAGES EDITOR

Douglas Henry Juers Whitman College

MANAGING EDITOR

Serena Tan, PhD Wiley

EDITORIAL ADVISORY BOARD

Ivet Bahar University of Pittsburgh

P. Balaram Indian Institute of Science

Maurizio Brunori Sapienza-University of Rome

Zengyi Chang Peking University

David Christianson University of Pennsylvania

Andrew J. Doig University of Manchester

Arne Elofsson Stockholm University

Elisabeth Sauer-Eriksson Umea° University

Mark Gerstein Yale University

Lila Gierasch University of Massachusetts

Yuji Goto Osaka University

Gerald Hazelbauer University of Missouri-Columbia

Yoshiki Higuchi University Hyogo

Mei Hong Iowa State University

Joel Janin Universite´ Paris - Sud

Andrzej Joachimiak Argonne National Laboratory

Neville Kallenbach New York University

Igor Kaltashov University of Massachusetts, Amherst

Olivier Lichtarge Baylor College of Medicine Andreas Matouschek Northwestern University

Stephen Matthews Imperial College

Hiroyuki Noji University of Tokyo

Michael W. Parker ACRF Rational Drug Discovery Centre

Kevin Plaxco University of California, Santa Barbara

Carol B. Post Purdue University

Ronald Raines University of Wisconsin-Madison

Daniel Raleigh Stony Brook University

Christina Redfield Oxford Centre for Molecular Sciences

Robert Russell EMBL, Heidelberg

Fred Salsbury Wake Forest University

Robert Sauer Massachusetts Institute of Technology

Brenda Schulman St. Jude Children’s Research Hospital

Yigong Shi Tsinghua University School of Medicine

Kevan Shokat University of California, San Francisco

Jeffrey Skolnick Georgia Institute of Technology

Brian Sykes University of Alberta

Dan S. Tawfik Weizmann Institute of Science

Jill Trewhella University of Sydney

Vladimir Uversky University of South Florida

James Wells University of California, San Francisco

Ann H. West University of Oklahoma

Keith Wilson University of York

Stephen Withers University of British Columbia

Cynthia Wolberger Johns Hopkins University School of Medicine

Todd Yeates University of California, Los Angeles

Xuejun Cai Zhang National Laboratory of Macromolecules Chinese Academy of Sciences

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TABLE OF CONTENTS

Editorial

265 Protein Science Best Paper awards to Chih-Chia (Jack) Su and Minttu Virkki

Brian W. Matthews

Reviews

267 Emerging applications of small angle solution scattering in structural biology

Barnali N. Chaudhuri

277 Role of membrane contact sites in protein import into mitochondria

Susanne E. Horvath, Heike Rampelt, Silke Oeljeklaus, Bettina Warscheid, Martin van der Laan, and Nikolaus Pfanner

298 The structural basis for specificity in lipoxygenase catalysis

Marcia E. Newcomer and Alan R. Brash

Articles

310 Specific ion effects on macromolecular interactions in Escherichia coli extracts

Ciara Kyne, Brian Ruhle, Virginie W. Gautier, and Peter B. Crowley

319 Molecular modeling and computational analyses suggests that the Sinorhizobium meliloti periplasmic regulator protein ExoR adopts a superhelical fold and is controlled by a unique mechanism of proteolysis

Scan the QR code with your smartphone to view Protein Science Video Highlights: summaries of featured Protein Science articles created by the articles’ authors

328 Structural features of interfacial tyrosine residue in ROBO1

fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analyses

Taisuke Nakayama, Eiichi Mizohata, Takefumi Yamashita, Satoru Nagatoishi, Makoto Nakakido, Hiroko Iwanari, Yasuhiro Mochizuki, Yuji Kado, Yuki Yokota, Reiko Satoh, Kouhei Tsumoto, Hideaki Fujitani, Tatsuhiko Kodama, Takao Hamakubo, and Tsuyoshi Inoue

341 Exploring the folding pathway of green fluorescent protein through disulfide engineering

Derek J. Pitman, Shounak Banerjee, Stephen J. Macari, Christopher A. Castaldi, Donna E. Crone, and Christopher Bystroff

354 Kinetics and thermodynamics of metal-binding to histone deacetylase 8

Byungchul Kim, Amit S. Pithadia, and Carol A. Fierke

366 Change in structure and ligand binding properties of hyperstable

cytochrome c₅₅₅ from Aquifex aeolicus by domain swapping

Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Yoshiki Higuchi, and Shun Hirota

376 Crystal structure of afadin PDZ domain–nectin-3 complex shows the structural plasticity of the ligand-binding site

Yoshie Fujiwara, Natsuko Goda, Tomonari Tamashiro, Hirotaka Narita, Kaori Satomura, Takeshi Tenno, Atsushi Nakagawa, Masayuki Oda, Mamoru Suzuki, Toshiaki Sakisaka, Yoshimi Takai, and Hidekazu Hiroaki

386 Crystal structure and functional analysis of MiD49, a receptor for the

mitochondrial fission protein Drp1

Oliver C. Losóon, Shuxia Meng, Huu Ngo, Raymond Liu, Jens T. Kaiser, and David C. Chan

395 Structural basis for acceptor-substrate recognition of UDP-glucose:

anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea

Takeshi Hiromoto, Eijiro Honjo, Naonobu Noda, Taro Tamada, Kohei Kazuma, Masahiko Suzuki, Michael Blaber, and Ryota Kuroki

408 Structure, activity, and stability of metagenome-derived glycoside hydrolase family 9 endoglucanase with an N-terminal Ig-like domain

Hiroyuki Okano, Eiko Kanaya, Masashi Ozaki, Clement Angkawidjaja, and Shigenori Kanaya

For the Record

420 K114 (trans, trans)-bromo-2,5-bis(4-hydroxystyryl)benzene is an efficient detector of cationic amyloid fibrils

Veli Selmani, Kevin J. Robbins, Valerie A. Ivancic, and Noel D. Lazo

426 C-terminal juxtamembrane region of full-length M2 protein forms a

membrane surface associated amphipathic helix

Shenstone Huang, Bryan Green, Megan Thompson, Richard Chen, Jessica Thomaston, William F. DeGrado, and Kathleen P. Howard

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