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. 2015 Feb 3;7(2):522–542. doi: 10.3390/v7020522

Figure 7.

Figure 7

Characterization of EHV-1_gBY336A. (a) ED cells were infected with the respective viruses at an MOI of 0.01. Means ± SD of diameters of 100 plaques measured for each virus are shown. The plaque diameter of parental viruses was set to 100%. No significant differences (one-way ANOVA; p > 0.05) between parental EHV-1 and EHV-1_gBY336A were evident; (b) One million peripheral blood mononuclear cells (PBMC) were incubated with parental and recombinant EHV-1 viruses at an MOI of 1 for 24 h at 37 °C. After incubation, the percentage of infected cells was determined by flow cytometry. The rate of infection of parental virus was set to 100%. All data represent the means ± SD of three independent experiments (Kruskal–Wallis one-way analysis of variance; p > 0.05). For single-step growth kinetics of EHV-1 recombinant viruses, ED cells were infected at an MOI of 0.1 (c,d), followed by citrate treatment (pH = 3) to remove remaining extracellular virions. Infected cells (c) and supernatants (d) were separately collected and virus titers were determined at the indicated times p.i. The data presented are means ± SD of three independent measurements. No significant differences were detectable for the EHV-1 recombinant viruses when compared to the parental viruses (Friedman test–Dunn’s multiple comparison test; p > 0.05).