Figure 2.

ECIS measurement of molecular weight-based fractions of patient plasma showing that proteins retained by 100 kDa cutoff filters cause permanent loss of resistance. Arrows indicate the time points when media was changed (19 h) and plasma samples were added (22 h). (B) Baseline normalized ECIS profiles shows the effect of plasma proteins fractionated by molecular weight on cell barrier function (in different sample from (A)). Component protein(s) retained by the 100 kDa filter are shown to cause sustained loss of resistance, while lower molecular weight proteins of 30 kDa and 10 kDa range are shown to show reversible loss of resistance.