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Overproduction of Ndc80 in S. pombe cells. The outer kinetochore Ndc80 complex is made up from Ndc80, Nuf2, Spc24 and Spc25. Under ‘overexpressed’ condition, two populations of Ndc80 will be formed. One propotion will be incorporated into the Ndc80 complex in place of endogenously produced Ndc80 and localise to the outer kinetochore, whereas the excess ‘free’ Ndc80 may be dispersed or form large polymers/aggregates in the cytoplasm.
