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. 2014 Dec 23;6(2):771–788. doi: 10.18632/oncotarget.2718

Figure 5. SKP2 inactivation induces an accumulation of ubiquitinated JARID1B in nucleolus of cells in vitro and in vivo for cellular senescence.

Figure 5

(A) Immunofluorescence (IF) images show a co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of PC3 cells upon SKP2 knockdown (Also see Supplementary Figure S6A). FBL indicates a nucleolar marker. Right panel: quantification of PC3 cells showing an increase of JARID1B localization in nucleolus. Error bars represent means ± SD. (B) IF images show a co-localization of endogenous JARID1B and K63-Ub in nucleoli of PC3 cells upon SKP2 knockdown. (C) Western blotting assay shows an increase of β-galactosidase (β-Gal) in PC3 cells upon SKP2 knockdown. (D) IF images show JARID1B in nucleolus as indicated by arrows and β-Gal in cytoplasm in senescent cells upon SKP2 knockdown. (E) The co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of prostate tissues in Ptenpc−/−;Trp53pc−/−;Skp2−/− mutant mice (Also see Supplementary Figure S6B). Scale bars represent 10 μm for panel A, B, D and E. (F) The positive staining of β-galactosidase in prostate tissues of Ptenpc−/−;Trp53pc−/−;Skp2−/− mice but not in that of Ptenpc−/−;Trp53pc−/− mice. Scale bars represent 50 μm.