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. 2014 Dec 22;6(3):1422–1434. doi: 10.18632/oncotarget.2805

Figure 1. WNK2 protein expression associates with reduced MMP2 expression and activity.

Figure 1

(A) MSP analysis of the WNK2 gene promoter in a panel of glioma cell lines. (B) The activity of MMP2 and MMP9 was analyzed by gelatin zymography using conditioned medium of glioma cell lines with and without WNK2 expression. C+, positive control for MMP2 and MMP9, conditioned medium of a gastric cell line stimulated with macrophages. (C) MMP2 and MMP9 mRNA levels were measured by qRT-PCR in A172 and SW1088 cell lines. Relative expression changes in SW1088 are presented as fold increase of MMP/GAPDH in comparison to A172 cells. Data on graphs represent the mean values ± standard errors and are representative of, at least, three independent experiments. ***, significantly different from A172 cells (p < 0,001).