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. 2015 Feb 12;6(3):1640–1651. doi: 10.18632/oncotarget.2746

Figure 1. Combination of Chal-24 and cisplatin results in synergistic apoptotic cell death in lung cancer cells.

Figure 1

(A) A549 cells were treated with cisplatin (10–30 μM) and Chal-24 (0.5 μM) for 48 h. Cell death were measured by LDH release assay. Data shown are mean ± SD, representative of three independent experiments. (B) A549 cells were treated with cisplatin (10 μM) and Chal-24 (0.125–1 μM) for 48 h. Cell death were measured as described in A. (C) A549 cells were treated with cisplatin (10 μM) and Chal-24 (1 μM) for indicated time periods. Cell extracts were resolved in 12% SDS-PAGE gels. PARP and active caspase-3 were detected by Western blot. GAPDH was detected as an input control. (D) A549 cells were pretreated with z-VAD (10 μM) for 30 min, followed by 48 h treatment with cisplatin (10 μM) and Chal-24 (1 μM), cell death were detected as described in A. (E) the cells were transfected with indicated siRNA for 24 h, and treated with (10 μM) and Chal-24 (1 μM) for additional 48 h. Cell death was measured as described in A. **p < 0.01. Insert, knockdown of caspase 9 expression was confirmed by Western blot.