Fig. 4.

Magi AJ localization is independent of RASSF8 and ASPP. (A-A‴) In larval wing imaginal disc epithelial cells Magi (green, A′) colocalizes with E-Cad (blue, A‴) at the AJ level, basal to aPKC (red, A″). A confocal z-section is shown. The boxed region is shown at higher magnification to the right. (B-C‴) Confocal z-sections of larval wing discs overexpressing GFP-tagged Magi (B) or MagiΔWW (C) using the ptc-Gal4 driver. Both Magi constructs (GFP, green, B′,C′) colocalize with E-Cad (blue, B‴,C‴), apical to the septate junction protein Dlg (red, B″,C″). (D-D″) In 24-h APF pupal eye discs, Magi (green, D′) is localized at cell junctions in IOCs together with E-Cad (red, D″). Scale bars: 20 µm in A-C; 10 µm in D.