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. Author manuscript; available in PMC: 2015 Jun 6.
Published in final edited form as: Neuroscience. 2014 Mar 26;269:67–78. doi: 10.1016/j.neuroscience.2014.03.014

Figure 6.

Figure 6

Immunohistochemical analysis of nNOS expression (green) in cholera toxin-B (CTB) labeled phrenic motor neurons (red) in the ~C4 spinal cord. Note the expression nNOS within CTB-positive neurons (double-labeled) and also neighboring cells (arrow). Low magnification also shows diffuse expression in the phrenic motor nucleus region; a higher magnification (inset) also shows expression specifically within phrenic motorneurons. Lower magnification represents are shown as a Z-stack (16 images, 2μm steps, rendered as max intensity; scale bar = 100μm) whereas higher magnification of the region of interest (inset) are a one stack image taken in the middle of the Z-stack to highlight nNOS expression within the cytoplasm (Scale bar = 10μm).