Vol. 207 No. 5, December 8, 2014. Pages 599–613.
In the original version of Fig. S2, the panel showing staining with anti-RAD51 in non-irradiated (0 Gy) Brca2 WT/WT cells was incorrect. A corrected version of Fig. S2 is shown below.
Figure S2.
RAD51 foci induction in Brca2GFP/GFP cells. Confocal microphotographs of wild-type and Brca2 GFP/GFP cells were stained by indirect immunofluorescence with anti-RAD51 antibody (red). BRCA2-GFP is visualized directly (green); the nuclei of wild-type cells imaged under the same settings emit low levels of background fluorescence. Nuclear DNA is stained with DAPI (blue). The number of RAD51 foci per confocal slice of a nucleus was determined in 30–35 randomly sampled nuclei per each condition. An arbitrary cutoff of 10 foci per nucleus was used to define positive cells. Bars, 10 µm.