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. 2014 Apr 18;26(2):154–163. doi: 10.3109/09537104.2014.898178

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© 2015 Informa UK Ltd.

This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of storage in additive solution or treatment with Irradiation, mirasol, or intercept on platelet microRNA synthesis. The figure shows the percentage of pre-microRNA substrate cleaved into mature microRNA by platelet Dicer, which is known to mediate platelet microRNA biogenesis [11]. Dicer activity assays were performed by coincubating a ³²P-labeled pre-microRNA substrate (³²P-labeled pre-let-7a-3) with protein extracts from platelets collected from PCs at 1, 4, and 7 days of storage after treatment with additive solution, Irradiation, mirasol, intercept, or no treatment (control). For each group and time point, the figure shows the individual data (n = 8 donors per group), along with their median.