Figure 5.

Effect of storage in additive solution or treatment with Irradiation, mirasol, or intercept on the ability of platelets to mediate microRNA function. The figure shows the percentage of miR-223 sensor RNA substrate cleaved by platelet Ago2•miR-223 complexes, which is known to mediate platelet microRNA function [11]. RISC activity assays were performed by coincubating a ³²P-labeled RNA sensor perfectly complementary to miR-223 with protein extracts from platelets collected from PCs at 1, 4, and 7 days of storage after treatment with additive solution, Irradiation, mirasol, intercept, or no treatment (control). For each group and time point, the figure shows the individual data (n = 4 donors per group), along with their median.