Fig. 6.

The Walker A motif of PglY is required for Pgl function. (A) Strains (indicated) were challenged with 1×10⁵ pfu ml⁻¹ of ϕC31cΔ25 harvested following J1929 infection (PglY⁻). (B) Release of Pi from ATP as a function of the PglY ATPase domain in wild type PglY and the PglY^K81A/S82A mutant. Wild-type PglY (squares) and PglY^K81A/S82A (circles). Data are mean of 3 replicate experiments. (C) Hydrolysis of ATP in the presence of ADP-NP. 4 mM ATP (black triangles; 3 mM ATP, 1 mM ATP-NP (circles); 2 mM ATP, 2 mM ATP-NP (grey triangles); 1 mM ATP, 3 mM ATP-NP (squares). Data are mean of three replicate experiments.